Plasmid_Backbone

Part:BBa_K268000:Design

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)

pSB6A0


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4793
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 46
    Illegal NotI site found at 4799
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4793
    Illegal BamHI site found at 28
    Illegal XhoI site found at 4760
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4793
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4793
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4808
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3387
    Illegal SapI.rc site found at 2365
    Illegal SapI.rc site found at 4469


Design Notes

  • Removal of non-biobrick compatible restriction sites.
  • Two origins of replication. Original plasmid allowed for bi-directional insertion of parts (either by f1 or ColE1 origin).
  • CEN6_ARS4 allows for plasmid expression in S. cerevisiae

Source

Unmodified version can be found by accession number U03440.

References